COVALENT IMMOBILIZATION OF GLUCOSE-OXIDASE ON POLY[1-(2-CARBOXYETHYL)PYRROLE] FILM FOR GLUCOSE SENSING

Poly[1-(2-carboxyethyl)pyrrole] film (PPy-COOH film) was electrochemic ally prepared and glucose oxidase (GOD) was covalently immobilized on the PPy-COOH him by the condensation reaction with carboxyl groups of the PPy-COOH. The apparent activity of the GOD immobilized on the PPy- COOH film was 152 mU cm(-2). The GOD-immobilized PPy-COOH film (PPy-GO D film) was applied to amperometric glucose sensing. Plots of response current versus glucose concentration gave a straight line with a slop e of 1.7 mu A cm(-2) per 1 mM glucose up to 80 mM. The glucose oxidati on with the GOD on the PPy-GOD film was considered to proceed through a Michaelis-Menten mechanism. (C) 1998 Elsevier Science Ltd. All right s reserved.