ASSAY FOR SIMULTANEOUS DETERMINATION OF BETA-CYCLODEXTRIN GLYCOSYLTRANSFERASE (BETA-CGTASE) AND AMYLASE

A new procedure was developed for the simultaneous determination of be ta-cyclodextrin glycosyltransferase (beta-CGTase) and amylase activiti es in Bacillus macerans cultures. The new amylase assay branched off f rom the beta-CGTase activity measurement according to Kaneko et al. (J . Jpn. Sec. Starch Sci. 34 [1987], 45-48), included water-soluble star ch as substrate and based on the well-characterized K-3[Fe(CN)(6)] red ucing sugar analysis procedure. The assay had excellent within-run and between-run CVs, and also had an excellent correlation with the widel y used 3,5-dinitrosalicylic acid method of Bernfeld (Meth. Enzymol. 1 [1955], 149-158) using Bacillus licheniformis a-amylase as a model enz yme at 50 degrees C. Neither high cyclodextrin glycosyltransferase act ivities nor the self-degradation of starch interfered with the new amy lase assay.