DISSOCIATION BETWEEN VOLUME BLOOD-FLOW AND LASER-DOPPLER SIGNAL FROM RAT MUSCLE DURING CHANGES IN VASCULAR TONE

Although the laser-Doppler flowmetry (LDF) signal. fi om skeletal musc le has been shown to provide a good measure of blood flow under some c onditions, its behavior during administration of vasoactive substances has never been addressed. The aims of this study were to compare Ii c hanges in LDF signal with those in total muscle blood flow measured wi th radioactive microspheres after ganglionic blockade (chlorisondamine ) and during administration of angiotensin II (ANG II), phenylephrine (PE), and isoproterenol (Iso) and 2) changes in vascular resistance es timated by the two techniques. The LDF signal from the biceps femoris muscle was investigated in anesthetized male Wistar rats. Ganglionic b lockade led to a significant (P < 0.05) fall in mean arterial pressure (MAP) [medians (lower, upper quartiles): 78 (72, 83) vs. 127 (114, 13 8) mmHg under basal conditions], muscle blood flow (MBF, microsphere t echnique; 61%), and the LDF signal (29%). Muscle vascular resistance ( MVR = MAP/MBF) was increased (64%, P < 0.05), but vascular resistance estimated as MAP/LDF signal (MVRLDF) was unchanged. During ANG II and PE infusions, MAP rose (P < 0.05) to 178 (155, 194) and 127 (124, 142) mmHg, respectively; MBF did not change compared with the preinfusion (postganglionic blockade) level and remained significantly (P < 0.05) lower than baseline, whereas the LDF signal increased up to a level no t different from baseline. MVR rose and was significantly (P < 0.05) h igher than baseline, whereas MVRLDF did not differ significantly from baseline. During Iso infusion, MAP fell [58 (56, 60) vs. 94 (92, 102) mmHg, P < 0.05], the LDF signal was reduced (49%, P < 0.05) despite a large increase in MBF (139%, P < 0.05), and MVR fell (74%, P < 0.05), whereas MVRLDF did not change vs. preinfusion level. Our results sugge st that Ii changes in the LDF signal from muscle may not correlate wit h changes in total muscle blood flow measured by the microsphere techn ique during infusion of vasoactive substances and 2) the use of LDF da ta for estimation of MVR during changes in vascular tone in rat skelet al muscle is probably not appropriate.