IN-VITRO STUDIES OF ENZYME-RELEASE FROM DERMAL PHARMACEUTICAL PRODUCTS

Using the enzyme collagenase as an example, we describe a model develo ped from the Michaelis-Menten and simple diffusion equations which per mits a quantitative determination of enzyme release from a carrier on the basis of photometric measurements. It is shown that the action of anhydrous topical pharmaceutical products containing collagenase, e.g Novuxol(R) or Iruxol(R), is determined not by release of the enzyme bu t by reactions at the collagenase/wound surface interface.