ON THE LONG-WAVELENGTH SPECTRAL FORMS OF CHLOROPHYLL-A IN PHOTOSYSTEM-I - SPECTROSCOPIC AND IMMUNOLOGICAL INVESTIGATIONS ON A GREENING MUTANT OF THE GREEN-ALGA SCENEDESMUS-OBLIQUUS

The origin of the long-wavelength chlorophyll(Chl) absorption (lambda( peak) > 680 nm) and fluorescence emission (lambda(peak) > 685 nm) has been investigated on Scenedesmus mutants (C-2A'-series, lacking the ab ility to synthesize chlorophyll in the dark) grown at 0.3 (LL), 10 (ML ) and 240 mu E s(-1) m(-2) (HL). LL cells are arrested in an early gre ening state; consequently, 'Chl availability' determines the phenotype . LL thylakoids are totally lacking long-wavelength Chl; nonetheless, PS I and PS II are fully functional. Gel electrophoresis and Western b lots indicate that four out of seven resolved LHC polypeptides seem to require a high Chi availability for assembly of functional chlorophyl l-protein complexes. The PS I core-complex of ML and HL thylakoids con tains long-wavelength chlorophylls, but in the PS I core-complex of LL thylakoids these pigments are lacking. We conclude that long-waveleng th pigments are only present in the PS I core in the case of high Chl availability. The following hypothesis is discussed: Chl availability determines not only the LHC polypeptide pattern, but also the number o f bound Chl molecules per individual pigment-protein complex. Chl-bind ing at non-obligatory, peripheral sites of the pigment-protein complex results in long-wavelength Chl. In the case of low Chl availability, these sites are not occupied and, therefore, the long-wavelength Chl i s absent.