We end-labeled Hin fI restriction digests of a PCR-amplified plastid e
ncoded gene, the large subunit of ribulose bisphosphate carboxylase, t
o investigate patterns of cpDNA inheritance in Turnera ulmifolia. A to
tal of 70 progeny from crosses among plants taken from ten populations
revealed varying patterns of inheritance. A majority of progeny inher
ited the paternal cpDNA (64%), while 19% exhibited maternal and 17% bi
parental inheritance. Eight variegated progeny showed biparental inher
itance and were analyzed in greater detail. We extracted and analyzed
the cpDNA content of light- vs. dark-green leaf sectors from these pla
nts. The results showed that vegetative segregation of cpDNA had occur
red for seven of the eight plants.