MOLECULAR-CLONING OF A CDNA-ENCODING A POLLEN EXTRACELLULAR PROTEIN AS A POTENTIAL SOURCE OF A POLLEN ALLERGEN IN BRASSICA-RAPA

Authors
TORIYAMA K HANAOKA K OKADA T WATANABE M
Citation
K. Toriyama et al., MOLECULAR-CLONING OF A CDNA-ENCODING A POLLEN EXTRACELLULAR PROTEIN AS A POTENTIAL SOURCE OF A POLLEN ALLERGEN IN BRASSICA-RAPA, FEBS letters, 424(3), 1998, pp. 234-238
Citations number
32
Categorie Soggetti
Biology,"Cell Biology",Biophysics
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
424
Issue
3
Year of publication
1998
Pages
234 - 238
Database
ISI
SICI code
0014-5793(1998)424:3<234:MOACAP>2.0.ZU;2-8
Abstract
A polyclonal antiserum was raised against the extracellular pollen pro teins of Brassica Papa and used for screening the expression cDNA libr aries made from immature anthers, We obtained five groups of cDNA clon es, including cDNAs similar to PCP1, thioredoxin, and lipid transfer p rotein (LTP), Recombinant protein of the cDNA clone showing sequence s imilarity to LTP was demonstrated to bind IgE of a patient allergic to Brassica pollen, The cDNA clone reported here, therefore, represents a novel pollen allergen of Brassica rapa. (C) 1998 Federation of Europ ean Biochemical Societies.