PROSTAGLANDIN AND PROGESTERONE PRODUCTION BY BOVINE LUTEAL CELLS INCUBATED IN THE PRESENCE OR ABSENCE OF THE ACCESSORY CELLS OF THE CORPUS-LUTEUM AND TREATED WITH INTERLEUKIN-1-BETA, INDOMETHACIN AND LUTEINIZING-HORMONE

This experiment examined production of prostaglandin (PG) F-2 alpha, P GE(2) and progesterone by bovine luteal cells incubated with or withou t the accessory cells and treated with recombinant bovine interleukin- 1 beta (10 ng), indomethacin (5 mu g) or luteinizing hormone (50 ng). During pretreatment, progesterone production was similar in the luteal and luteal plus accessory cells, being greater in either of these tha n in accessory cells; PGF(2 alpha) was greatest in luteal plus accesso ry, intermediate in accessory and lowest in the luteal cells; PGE(2) w as similar among all cell arrangements. Luteinizing hormone or luteini zing hormone plus indomethacin stimulated progesterone in the luteal a nd luteal plus accessory cells, this being similar in these two cell a rrangements. Interleukin-1 beta stimulated PGF(2 alpha) in luteal and luteal plus accessory cells, and tended to elevate PGF(2 alpha) in acc essory cells. Interleukin-1 beta stimulated PGE(2) in all cell arrange ments. Indomethacin inhibited the interleukin-1 beta-stimulated PGF(2 alpha) and PGE(2) release. Indomethacin or indomethacin plus luteinizi ng hormone inhibited basal PGE(2) in luteal and luteal plus accessory cells. These data suggest that progesterone production is not influenc ed by the presence of accessory cells of the corpus luteum, that acces sory and luteal cells produce appreciable amounts of PG, and that reco mbinant bovine interleukin-1 beta stimulates PGE(2) and PGF(2 alpha) i n the luteal and accessory cells.