Tissue culture techniques have an important role in the genetic improv
ement of crop plant species. However, genomic instability, in vitro re
calcitrance, and loss of morphogenetic potential are limiting factors
affecting plant biotechnology initiatives. The objectives of this revi
ew are to evaluate the experimental evidence for the occurrence of fre
e radical processes in plant tissue cultures. Relationships between ox
idative status and in vitro plant development will be assessed and the
implications that these factors may have for crop plant biotechnology
explored Achieving a better understanding of free radical mechanisms
in tissue cultures may have useful applications in crop plant improvem
ent.