AN EVALUATION OF ANALYTICAL METHODS FOR THE DETECTION OF SECONDARY OXIDATION-PRODUCTS IN DEDIFFERENTIATED PLANT CULTURES

A range of techniques have been employed to detect and examine aldehyd ic products of lipid peroxidation in D. carota cultures. Emphasis is p laced on the use of HPLC and LC-MS techniques to aid the identificatio n and profiling of aldehydic dinitrophenyl hydrazone (DNPH)-derivative s in dedifferentiated callus tissues. Using these approaches, malondia ldehyde (MDA) and 4-hydroxynonenal (HNE) have been identified in cultu res of D. carota. Using LC-MS, it was found that embryogenic cultures have high levels of HNE and lower levels of MDA compared to nonembryog enic cultures. Future studies will evaluate the role of these lipid pe roxidation products in in vitro morphogenesis.