CONSEQUENCES OF PHOTOOXIDATION IN TRANSGENIC TOBACCO WITH CO-SUPPRESSION OF COPROPORPHYRINOGEN OXIDASE

Citation
Hp. Mock et al., CONSEQUENCES OF PHOTOOXIDATION IN TRANSGENIC TOBACCO WITH CO-SUPPRESSION OF COPROPORPHYRINOGEN OXIDASE, Phyton, 37(3), 1997, pp. 169-174
Citations number
13
Journal title
PhytonACNP
ISSN journal
00792047
Volume
37
Issue
3
Year of publication
1997
Pages
169 - 174
Database
ISI
SICI code
0079-2047(1997)37:3<169:COPITT>2.0.ZU;2-T
Abstract
Introduction of additional copies of genes encoding coproporphyrinogen oxidase (CPO) caused in some transgenic lines necrotic leaf lesions. As a result of mutual silencing of transgenes and endogenous genes CPO activities were reduced and photosensitive coproporphyrin(ogen) accum ulated. Photo-oxidation of these porphyrins generated reactive oxygen species which induced the protection mechanism against oxidative stres s. Activities of ascorbate peroxidase, glutathione reductase and super oxide dismutase were increased in these transgenic lines. We conclude that these plants accumulating porphyrin are appropriate for studies o n the induction mechanisms of the antioxidative defense system.