ENHANCED DETECTION OF LIPOPROTEIN-LIPASE BY COMBINING IMMUNOPRECIPITATION WITH WESTERN-BLOT-ANALYSIS

This manuscript describes the problems inherent in combining immunopre cipitation of lipoprotein lipase (LPL) with its detection by Western b lot, and how these problems can be circumvented by the preparation of suitable immunoreagents. These reagents used during the immunoprecipit ation step, include Fab fragments of the primary antibody (chicken ant i-bovine LPL), and a covalently linked immunomatrix of the secondary a ntibody (rabbit anti-chicken Igc). The use of these reagents in conjun ction with Western blot detection virtually eliminates the problem of non-relevant protein detection when analyzing LPL from complex biologi cal samples. Moreover, this approach can be adapted to detect any prot ein with the same inherent problems as LPL, such as hepatic lipase.