DIFFERENTIATION OF OLIGODENDROCYTE OCCURS IN CONTACT WITH ASTROCYTE

Citation
Y. Sakurai et al., DIFFERENTIATION OF OLIGODENDROCYTE OCCURS IN CONTACT WITH ASTROCYTE, Journal of neuroscience research, 52(1), 1998, pp. 17-26
Citations number
38
Categorie Soggetti
Neurosciences
ISSN journal
03604012
Volume
52
Issue
1
Year of publication
1998
Pages
17 - 26
Database
ISI
SICI code
0360-4012(1998)52:1<17:DOOOIC>2.0.ZU;2-L
Abstract
We have generated stage-specific oligodendrocytes, which are identifie d as immature oligodendrocytes by the expression of specific markers. These cells were generated by using 3 types of intrinsic and extrinsic factors for influencing the characteristics of oligodendrocyte-lineag e cells, The factors included 10% serum in the medium to induce cell p roliferation, bFGF in serum-free medium to increase survival and induc e differentiation, and 0.25% trypsin to remove nonoligo dendrocyte lin eage cells. We have also demonstrated that it is possible to supply a huge amount of homogeneous populations of stage-specific immature olig odendrocytes after 35 days' culture. The maturation of these cells is controlled by interacting with astrocytes in vitro, resulting in the e xpression of MBP isoforms (14.0 and 18.5 kDa) that may participate in mature oligodendrocyte membrane compaction. (C) 1998 Wiley-Liss, Inc.