THE EFFECT OF TYROSINE KINASE INHIBITORS ON IGE-MEDIATED HISTAMINE-RELEASE FROM HUMAN LUNG MAST-CELLS AND BASOPHILS

Objective and Design: To investigate the role of tyrosine kinases (TK) in IgE-mediated signal transduction in human lung mast cells (HLMC) a nd basophils. Materials: Peripheral blood basophils (n greater than or equal to 4) and human lung mast cells (n greater than or equal to 6). Treatment: Cells were preincubated with TK inhibitor for 15 min at 37 degrees C, before the addition of anti-IgE. Methods: Histamine releas e (HR) was assayed using a fluorimetric technique. Results were compar ed using nonparametric statistics. Results: Piceatannol and ST638 sign ificantly (p less than or equal to 0.05) inhibited anti-IgE induced HR from HLMCs and basophils whilst lavendustin C had no effect in either cell type. Herbimycin A also significantly (p less than or equal to 0 .05) inhibited anti-IgE induced HR from both cell types, an effect whi ch was dose dependent but did require a 16 h preincubation with drug. Conclusions: In summary, HLMCs and basophils exhibit distinct inhibito ry profiles in the presence of various inhibitors of TK.