Pw. Stoecker et al., DETERMINATION OF GALACTOSE AND GALACTOCEREBROSIDE USING A GALACTOSE-OXIDASE COLUMN AND ELECTROCHEMICAL DETECTOR, Analytical biochemistry, 258(1), 1998, pp. 103-108
Citations number
20
Categorie Soggetti
Biology,"Biochemical Research Methods","Chemistry Analytical
A method has been developed to measure galactose and galactocerebrosid
e using galactose oxidase immobilized on a solid resin. Galactose oxid
ase converts galactose and galactocerebroside to their corresponding a
ldehydes and hydrogen peroxide, the latter being electroactive and mea
surable by electrochemical detection using DC amperometric detection,
The minimal detection limits of galactose and galactocerebroside were
1 and 2 mu M, respectively. The linear response to galactose and galac
tocerebroside was to at least 300 mu M. About 100 samples can be measu
red per hour using flow injection analysis. The activity of sulfatidas
e (cerebroside-3-sulfate-3-sulfohydrolase), which converts sulfatide (
sulfogalactocerebroside) to galactocerebroside, was measured, and its
inhibition by O-phospho-L-tyrosine was determined. (C) 1998 Academic P
ress.