ACTIVATED PROTEIN-C RESISTANCE ASSAY AS A SCREENING-TEST FOR THROMBOEMBOLIC DISPOSITION

An activated partial thromboplastin time (APTT) based method was devel oped to determine the anticoagulant response in patient plasma to adde d purified activated protein C (APC). On the other hand, Bertina et al . (1994) reported that one point mutation of amino acid 506 in the fac tor V was observed in such APC resistance patients. APC resistance for blood samples with known abnormal findings on the coagulation test al so may be determined. Coagmaster II for the APTT assay system (Sankyo, Tokyo, Japan) was employed. The APC resistance assay system was a use ful reagent as a screening test for lupus anticoagulant (LA), in addit ion to APC resistance.