HELICOBACTER-PYLORI LIPOPOLYSACCHARIDE INHIBITION OF GASTRIC SOMATOSTATIN RECEPTOR - EFFECT OF SUCRALFATE

In this study, we investigated the inhibitory effect of Helicobacter p ylori lipopolysaccharide on the binding of somatostatin to gastric epi thelial cell membrane receptor, and assessed the effect of antiulcer a gent, sucralfate, on this process. The assays conducted with the somat ostatin receptor, purified from the solubilized epithelial cell membra nes by affinity chromatography on Affi-Gel-[D-Tryp(8)]SRIF-14, reveale d a dose-dependent inhibition in receptor-somatostatin binding by the lipopolysaccharide which reached a maximum of 94.1%. This effect of H. pylori lipopolysaccharide was countered by sucralfate that produced a 92.5% restoration in receptor-somatostatin binding at 70 mu g/ml of t he drug. The findings demonstrate that sucralfate is capable of revers ing the interference by H. pylori lipopolysaccharide with somatostatin -receptor binding on gastric mucosal G-cells.