A METABOLIC MODEL DESCRIBING GROWTH AND SUBSTRATE UPTAKE OF TRIGONOPSIS-VARIABILIS

As a part of the study on the production of the enzyme D-Amino acid ox idase (D-AAO) by the yeast Trigonopsis variabilis, a chemically struct ured model capable of explaining cellular growth and substrate uptake of T. variabilis is presented. The model makes use of a simple sequenc e of biochemical reactions produced inside the cell and is capable of simulating a range of metabolic responses obtained front the T. variab ilis growth in discontinuous culture. The model can serve to understan d the behavior of the yeast under different growth conditions and coul d be used as a basis for rile design of an industrial fermentor for D- AAO production. Since some modifications have been introduced to the b asic metabolic model to provide even more generality, this new model c an be applied to any yeast growing under discontinuous conditions. (C) 1998 Elsevier Science Inc.