QUALITATIVE AND QUANTITATIVE-EVALUATION OF BISINDOLE USAMBARANE ALKALOIDS IN STRYCHNOS-USAMBARENSIS ROOTS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY DIODE-ARRAY

Authors
FREDERICH M TITS M ANGENOT L
Citation
M. Frederich et al., QUALITATIVE AND QUANTITATIVE-EVALUATION OF BISINDOLE USAMBARANE ALKALOIDS IN STRYCHNOS-USAMBARENSIS ROOTS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY DIODE-ARRAY, Phytochemical analysis, 9(2), 1998, pp. 63-66
Citations number
11
Categorie Soggetti
Biology,"Chemistry Analytical","Plant Sciences
Journal title
Phytochemical analysis → ACNP
ISSN journal
09580344
Volume
9
Issue
2
Year of publication
1998
Pages
63 - 66
Database
ISI
SICI code
0958-0344(1998)9:2<63:QAQOBU>2.0.ZU;2-N
Abstract
A high performance liquid chromatographic method for the simultaneous determination of bisindole usambarane alkaloids from the roots of Stry chnos usambarensis using a diode-array detector is proposed, The syste m, consisting of a RP-8 Select B column and a linear gradient elution with acetonitrile and acetate buffer allowed the separation of all the alkaloids in 20 min, Usambarensine was found to be the major tertiary alkaloid present in the roots, In order to demonstrate the possibilit y of quantifying the bisindole alkaloids in crude roots extracts, the quantitative evaluation of 3',4'-dihydrousambarensine, usambarensine a nd N-b-methylusambarensine was carried out. (C) 1998 John Wiley & Sons , Ltd.