IDENTIFICATION OF TARGET-CELLS BY IMMUNOHISTOCHEMICAL DETECTION OF COVALENTLY REARRANGED ESTRADIOL IN REHYDRATED PARAFFIN SECTIONS

Authors
JUNGBLUT PW SIERRALTA WD
Citation
Pw. Jungblut et Wd. Sierralta, IDENTIFICATION OF TARGET-CELLS BY IMMUNOHISTOCHEMICAL DETECTION OF COVALENTLY REARRANGED ESTRADIOL IN REHYDRATED PARAFFIN SECTIONS, HISTOCHEM C, 109(4), 1998, pp. 295-300
Citations number
15
Categorie Soggetti
Cell Biology
Journal title
HISTOCHEMISTRY AND CELL BIOLOGY
ISSN journal
09486143 → ACNP
Volume
109
Issue
4
Year of publication
1998
Pages
295 - 300
Database
ISI
SICI code
0948-6143(1998)109:4<295:IOTBID>2.0.ZU;2-8
Abstract
Estradiol is released from the binding niche of the receptor and coval ently arrested in the molecular vicinity by the Mannich reaction durin g target fixation in acetic acid/formaldehyde. The exposed steroid is freely accessible for appropriate antibodies. It can be visualized in sections by the second antibody/enzyme technique in high resolution an d without enhancements.