METAPHASE FRAGILITY OF THE HUMAN RNU1 AND RNU2 LOCI IS INDUCED BY ACTINOMYCIN-D THROUGH A P53-DEPENDENT PATHWAY

Infection of human cells with adenovirus 12 (Ad12), but not Ad2 or 5, induces four specific sites of metaphase chromosome fragility: the U1 small nuclear RNA (snRNA) genes (the RNU1 locus), the U2 snRNA genes ( RNU2), the U1 snRNA pseudogenes (PSU1) and the 5S rRNA genes (RN5S). S ignificantly, each of these sites corresponds to a multigene family en coding a small, abundant structural RNA. We and others have shown prev iously that Ad12-induced fragility of the RNU2 locus requires U2 snRNA promoter elements, viral early functions and p53 function, but not vi ral replication or integration, Rb function or chromosomal sequences f lanking the RNU2 locus. Remarkably, we now find that very low doses of actinomycin D (5-50 ng/ml) can phenocopy Ad12 infection: metaphase fr agility of the RNU1 and RNU2 loci is induced specifically in the absen ce of virus, and induction also requires U2 promoter elements and p53 function. Concurrently, it has been found by others that treatment wit h cytosine arabinoside (araC), but not aphidicolin, can also phenocopy Ad12 infection. We propose that Ad12 infection, actinomycin D and ara C all induce a similar or identical global damage arrest signal (perha ps a modification or altered conformation of p53) that preferentially interferes with metaphase condensation of the RNU1 and RNU2 loci. The RNU1 and RNU2 loci could be especially sensitive to this global signal either because specialized U snRNA transcription factors interact uni quely with the signal, or because the high concentration of short, act ive transcription units hinders chromatin condensation.