AN ORIGIN OF TRANSFER (ORIT) ON THE CONJUGATIVE ELEMENT PRS01 FROM LACTOCOCCUS-LACTIS SUBSP LACTIS ML3

Authors
MILLS DA PHISTER TG DUNNY GM MCKAY LL
Citation
Da. Mills et al., AN ORIGIN OF TRANSFER (ORIT) ON THE CONJUGATIVE ELEMENT PRS01 FROM LACTOCOCCUS-LACTIS SUBSP LACTIS ML3, Applied and environmental microbiology, 64(4), 1998, pp. 1541-1544
Citations number
29
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
64
Issue
4
Year of publication
1998
Pages
1541 - 1544
Database
ISI
SICI code
0099-2240(1998)64:4<1541:AOOT(O>2.0.ZU;2-N
Abstract
Previous analysis of the Tra1 region of the conjugative element pRS01 from Lactococcus lactis subsp, lactis ML3 suggested that an origin of transfer (oriT) was present, Deletion derivatives of this cloned Tra1 region were assayed for mobilization in the presence of the wild-type pRS01 element in trans, The pRS01 oriT was localized to a 446-nucleoti de segment in the intergenic region between open reading frames ltrD a nd ltrE. Sequence analysis of this region revealed a cluster of direct and inverted repeat structures characteristic of oriT regions associa ted with other conjugative systems.