TRANSFORMATION OF ACINETOBACTER SP. STRAIN BD413 BY TRANSGENIC SUGAR-BEET DNA

Authors
GEBHARD F SMALLA K
Citation
F. Gebhard et K. Smalla, TRANSFORMATION OF ACINETOBACTER SP. STRAIN BD413 BY TRANSGENIC SUGAR-BEET DNA, Applied and environmental microbiology, 64(4), 1998, pp. 1550-1554
Citations number
32
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
64
Issue
4
Year of publication
1998
Pages
1550 - 1554
Database
ISI
SICI code
0099-2240(1998)64:4<1550:TOASSB>2.0.ZU;2-T
Abstract
The ability of Acinetobacter sp. strain BD413(pFG4 Delta nptII) to tak e up and integrate transgenic plant DNA based on homologous recombinat ion was studied under optimized laboratory conditions. Restoration of nptII, resulting in kanamycin-resistant transformants, was observed wi th plasmid DNA, plant DNA, and homogenates carrying the gene nptII. Mo lecular analysis showed that some transformants not only restored the 317-bp deletion but also obtained additional DNA.