J. Lodowska et al., CHANGES IN FREE FATTY-ACID COMPOSITION IN CHLORELLA-VULGARIS AT VARIOUS STAGES OF THE LIFE-CYCLE, Acta Biologica Cracoviensia. Series Botanica, 39, 1997, pp. 91-97
The aim of the presented work was to determine and calculate the free
fatty acid (FFA) total concentration at various stages of Chlorella vu
lgaris cells cultivated synchronously. The FFA were isolated from cell
s harvested at given life cycle stages by the use of the SPE method, m
ethylated to methyl esters and analyzed qualitatively and semiquantita
vely by GC/MS. The total FFA concentration decreased during the time c
ourse of cultivation, the amount of unsaturated FFA decreased, and the
concentration of saturated FFA increased. The highest concentration w
as shown for C16:0. Statistical analysis showed that FFA at various st
ages of the Chlorella vulgaris life cycle may be grouped in classes wh
ich demonstrate similar changes in concentrations when observed during
the whole life cycle.