CHARACTERIZATION OF WHEAT-TRITICALE DOUBLED HAPLOID LINES BY CYTOLOGICAL AND BIOCHEMICAL MARKERS

Five wheat-triticale doubled haploid (DH) lines - M08, V209, DH220-14- 2, DH696-3-4 and M16 - derived from anther culture of F(1)s resulting from crosses involving hexaploid or octoploid triticale x hexaploid wh eat, were characterized by cytological and biochemical markers. Cytolo gical evidence from genomic in situ hybridization and C-banding indica ted that DH lines M08 and V209 (2n = 42) each contained a pair of 1BL/ 1RS translocation chromosomes. DH220-14-2 (2n = 42) was also a translo cated line with two pairs of chromosomes containing small fragments of rye. One of the translocation fragments carried the Sec-1R gene origi nating from the satellite region of 1RS; the origin of the other one r emains unknown. DH696-3-4 (2n = 42) contained a 3D(3R) substitution. I n M16 (2n = 44), three pairs of rye chromosomes, 3R, 4R and 6R, were p resent, 4R as an addition and 3D(3R) and 6D(6R) as substitutions. Bioc hemical, isozyme and storage protein markers confirmed the cytological conclusions. The advantages of transferring alien chromosomes or chro mosome fragments into wheat and creating alien aneuploid lines by anth er culture of hybrid F(1)s are discussed.