MOVEMENT OF VILLI INDUCES ENDOCYTOSIS OF NK1 RECEPTORS IN MYENTERIC NEURONS FROM GUINEA-PIG ILEUM

Agitation of villi evokes reflexes that affect the motility of the gui nea-pig small intestine. NK1 receptor endocytosis was used to investig ate the possible involvement of tachykinins acting on neuronal NK1 rec eptors in these reflexes, Segments of guinea-pig ileum were incubated at 37 degrees C in Krebs physiological saline containing 3x10(-6) M ni cardipine, with or without agitation of the villi by gas bubbles. Gut segments were fixed after 0-75 min and processed for immunohistochemis try to reveal the NK1 receptors, following which cells were imaged by confocal microscopy. Initially, receptors were located on the surface and in the cytoplasm of myenteric neurons. In gut incubated without mo vement of the villi, NK1 receptors returned to the cell surface. After 45 and 60 min, NK1 receptors were detected almost exclusively at the cell surface of 83% and 97% (respectively) of nerve cells that were im munoreactive for NK1 receptors and only 12%-13% of the NK1 receptor fl uorescence was located in the cytoplasm. Following the return of recep tor to the cell surface, agitation of the villi caused a new wave of e ndocytosis of the NK1 receptors in 70%-80% of the NK1 receptor-immunor eactive neurons. The percentage of the NK1 receptor fluorescence that was in the cytoplasm increased more than 2-fold to 27+/-2% after 15 mi n villous agitation. Action potential blockade by tetrodotoxin (3x10(- 7) M) prevented the internalisation of the NK1 receptor in response to villous agitation. The degree of internalisation caused by bubbling w as similar to that caused by 2x10(-9) M substance P. These results ind icate that, when enteric reflex circuits are activated by villous move ment, tachykinins are released and cause endocytosis of the NK1 recept or in a subpopulation of myenteric neurons.