THE STATISTICAL EVALUATION OF MIGRATION PARAMETERS OF FLAVONOIDS IN CAPILLARY ELECTROPHORESIS WITH REFERENCE TO STRUCTURAL DESCRIPTORS

Under each of 38 buffer conditions, six migration parameters of 14 fla vonoids were obtained in capillary zone electrophoresis (CZE) and nine parameters in micellar electrokinetic capillary chromatography (MEKC) . A total of 183 structural descriptors for each flavonoid were calcul ated to represent the molecular structures in a quantitative way. The relationship between the structural descriptors and the migration para meters of flavonoids in CZE or MEKC were evaluated by cluster and fact or analyses. Under all CZE conditions the apparent solute mobility (mu (a)) was always the first variable to associate with all molecule-leve l structural descriptors and the electrotopological state indices (Si) of skeletal carbons, whereas the effective mobility (mu e) was closer to Si of osi gens on skeletons. On the other hand, in MEKC retention factor or its logarithm did not depended more on the structural descri ptors than other migration parameters. This indicated that the paramet er did not represent the properties of the flavonoids better than othe r parameters. Thus, the results confirmed that hydrophobic interaction was not the only underlying force that influenced the migration behav iour of the flavonoids in MEKC.