UTILITY OF ION-PAIR CHROMATOGRAPHY FOR ANALYSIS OF SOME ANTHRACYCLINES IN PLASMA AND URINE

A high performance liquid chromatographic assay was developed and vali dated for a simultaneous determination of adriamycin and its metabolit es adriamycinol and adriamycinone as well as daunomycin in plasma. Ace tonitrile and trichloroacetic acid were employed in removing proteins and extracting the drugs and metabolites into the supernatant for HPLC . Enhancement of chemical stability of the anthracyclines during sampl ing procedure was investigated using 0.01 M gamma-cyclodextrin The pro cessed samples were chromatographed using an ODS/TM column (150 x 4.6 mm LD.) eluted with a mobile phase composed of 35% acetonitrile and 65 % (0.1M) monobasic phosphate, containing 0.3% heptafluorobutyric acid PH 3.