Qc. Chen et al., DETERMINATION OF BENZOYL PEROXIDE IN WHEAT-FLOUR BY ION CHROMATOGRAPHY WITH PRECOLUMN DERIVATIZATION, Journal of liquid chromatography & related technologies, 21(5), 1998, pp. 705-716
Citations number
18
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
A novel method for the determination of benzoyl peroxide bleach in whe
at flour by using high performance anion exchange chromatography (HPIC
) after decomposition to benzoic acid was proposed. Benzoyl peroxide w
as effectively extracted from wheat flour with absolute ethanol by ult
rasonication under neutral condition and converted to benzoic acid in
the presence of potassium hydroxide solution. The chromatographic sepa
ration of benzoic acid was performed on a Dionex OmniPac PAX-100 separ
ation column and the determination by ultraviolet absorbance detection
at 222 nm. Good linearity between the concentration of benzoyl peroxi
de and peak area response nas achieved in the range of 0.12-20 mu g/mL
. The detection limit (signal-to-noise ratio 3:1) was 19 ng/mL for ben
zoyl peroxide. The method has been successfully applied for the analys
is of real samples, and the average recoveries for various samples ran
ged from 81 to 92%.