RAPID EXTRACTION HPLC AS A TOOL FOR PRESUMPTIVE IDENTIFICATION OF BURKHOLDERIA-GLADIOLI, B-GLUMAE AND B-PLANTARII CAUSAL AGENTS OF VARIOUS RICE DISEASES

Authors
MATSUYAMA N UEDA Y IIYAMA K FURUYA N URA H KHAN AA MATSUMOTO M
Citation
N. Matsuyama et al., RAPID EXTRACTION HPLC AS A TOOL FOR PRESUMPTIVE IDENTIFICATION OF BURKHOLDERIA-GLADIOLI, B-GLUMAE AND B-PLANTARII CAUSAL AGENTS OF VARIOUS RICE DISEASES, Journal of the Faculty of Agriculture, Kyushu University, 42(3-4), 1998, pp. 265-272
Citations number
16
Categorie Soggetti
Agriculture
Journal title
Journal of the Faculty of Agriculture, Kyushu University → ACNP
ISSN journal
00236152
Volume
42
Issue
3-4
Year of publication
1998
Pages
265 - 272
Database
ISI
SICI code
0023-6152(1998)42:3-4<265:REHAAT>2.0.ZU;2-Y
Abstract
Rapid extraction-HPLC was performed to estimate its usefulness for pre sumptive identification of Burkholderia gladioli, B. glumae and B. pla ntarii. On HPLC chromatograms of 12 isolates of B. gladioli, a distinc t peak appeared at Rt 6.2 min without exception. This peak was absent on the chromatograms for 18 isolates of B. glumae and 5 isolates of B. plantarii tested. Benchmark peak at Rt 3.2 min was observed on the ch romatogram of B. plantarii, Together with the direct colony TLC method , this simplified HPLC analysis will be useful for identification of p hytopathogenic bacteria.