GROWTH-STIMULATION OF PEDIOCOCCUS SP. ISK-1 BY SAKEKASU (SAKE FILTER CAKE) AS A SUBSTITUTE FOR MEVALONIC ACID

Pediococcus sp. ISK-1, isolated in our laboratory, has been found to i nhabit in well-aged Nukadoko which is believed to have lasted for 360 gears. Vie compared the influence of ethanol between Lactobacillus hum ohiochii JCM 1199(T) and strain ISK-1 and found that the growth of str ain ISK-1 was seriously inhibited by ethanol. However, fermentation of ISK-1 was strongly stimulated by Yamahai-moto Sake and Sakekasu (Sake Filter Cake). Yamahai-moto Sake is obtained from traditional brewing, therefore, it was possible that Sakekasu would have been used for the first Nukadoko preparation at that time. Addition of 8.0% NaCl and 2. 0% Sakekasu in MRS broth resulted in substantially improving the cell population and lactate formation. Based on those facts, it is presumed that the first Nukadoko may have been prepared by the supplementation of Sakekasu, Soy souce, and Sail with selective pressure resulting in enrichment of strain ISK-1 of the bacteriocin producer.