The effect of different concentration of K+ and Na+ on the skin colour
of red sea bream and on melanosome movements in melanophores was inve
stigated by using a video microscope system and subsequent image analy
sis. Soaking in artificial sea water containing 470 mmol/L NaCl, 10 mm
ol/L KCl, 10 mmol/L CaCl2 and 50 mmol/L MgCl2 failed to prevent skin d
arkening, while solutions containing over 300 mmol/L KCI instead of th
e corresponding amount of NaCl induced aggregation of melanosomes, eff
ectively preventing skin darkening. However, soaking in a solution con
taining 480 mmol/L KCI diminished the red colour of the fish skin. It
is concluded that tile solution containing 300 mmol/L KCl is useful to
maintain skin colour of red sea bream and improve its commercial valu
e. (C) 1998 Academic Press Limited.