Mn. Jones et al., ANTIBACTERIAL REACTIVE LIPOSOMES ENCAPSULATING COUPLED ENZYME-SYSTEMS, International journal of pharmaceutics, 162(1-2), 1998, pp. 107-117
Liposomes have been prepared from phospholipid mixtures of dipalmitoyl
phosphatidylcholine (DPPC) and phosphatidylinositol (PI) encapsulating
the enzymes chloroperoxidase (CPO) and lactoperoxidase (LPO) in combi
nation with glucose oxidase (GO) by both extrusion (VET) and/or revers
e phase evaporation (REV). The liposomes were characterised in terms o
f the protein content and activity of the encapsulated enzymes. The an
tibacterial activity of these reactive liposomes arises from hydrogen
peroxide and oxyacids produced in the presence of the substrates gluco
se and chloride or thiocyanate ions. The liposomes were targeted to bi
ofilms of Steptococcus gordonii, an oral bacterium and their antibacte
rial activity was measured both as a function of liposome-biofilm incu
bation time and incubation time with the substrates. Bacterial inhibit
ion increases with both liposome-biofilm and substrate-biofilm incubat
ion time and with the extent of enzyme encapsulation. The reactive lip
osomes also display antibacterial activity in the presence of saliva.
The reactive liposomes have potential value in the context of oral hyg
iene. (C) 1998 Elsevier Science B.V. All rights reserved.