Y. Denizot et al., ARACHIDONIC-ACID AND HUMAN BONE-MARROW STROMAL CELLS, Biochimica et biophysica acta. Molecular cell research, 1402(2), 1998, pp. 209-215
Human bone marrow stromal cells regulate the growth of mal-row hematop
oietic progenitors by secreting cytokines. Arachidonic acid (AA) is th
e fatty acid precursor of prostaglandin E-2 (PGE(2)) and leukotriene B
-4 (LTB4) that modulate the growth of human bone marrow progenitors. W
e have investigated the incorporation of AA in human bone marrow strom
al cell cultures, their production of PGE(2) and LTB4 and the effect o
f AA on their growth. Gas chromatography analysis reveals the presence
of AA in the human bone marrow plasma and in bone marrow stromal cell
cultures. In stromal cells, [H-3]-AA is incorporated into triglycerid
es and is later delivered into phospholipids. Prelabeling-chase experi
ments indicate a preferential incorporation of AA into phosphatidyleth
anolamine and no trafficking of labeled AA between phospholipid specie
s. Bone marrow stromal cells release PGE(2) and LTB4 in response to ph
orbol myristic acetate (PMA) (1 mu M) and tumor necrosis factor alpha
(TNF-alpha) (10 ng/ml). Exogenous AA (up to 1 mu M) has no significant
effect on cell growth. In conclusion, human bone marrow stromal cells
capt exogenous AA and, thus, may participate to the control of marrow
AA concentrations. They may also regulate human marrow hematopoiesis
by secreting AA metabolites such as PGE(2) and LTB4. (C) 1998 Elsevier
Science B.V.