S. Schwank et al., IMPACT OF BACTERIAL BIOFILM FORMATION ON IN-VITRO AND IN-VIVO ACTIVITIES OF ANTIBIOTICS, Antimicrobial agents and chemotherapy, 42(4), 1998, pp. 895-898
The impact of bacterial adherence on antibiotic activity was analyzed
with two isogenic strains of Staphylococcus epidermidis that differ in
the features of their in vitro biofilm formation. The eradication of
bacteria adhering to glass beads by amikacin, levofloxacin, rifampin,
or teicoplanin was studied in an animal model and in a pharmacokinetic
ally matched in vitro model. The features of S. epidermidis RP62A that
allowed it to grow on surfaces in multiple layers promoted phenotypic
resistance to antibiotic treatment, whereas strain M7 failed to accum
ulate, despite initial adherence on surfaces and growth in suspension
similar to those for RP62A. Biofilms of S. epidermidis M7 were better
eradicated than those of strain RP62A in vitro (46 versus 31%; P < 0.0
5) as well as in the animal model (39 versus 9%; P < 0.01).