A NEW CATALASE ENZYME SENSOR ABLE TO DETERMINE THE HYDROGEN-PEROXIDE DIRECTLY IN CHLOROFORM

A new biosensor able to determine the hydrogen peroxide in water-satur ated or anhydrous chloroform, operating directly dipped in non-aqueous solutions was prepared and characterized. The biosensor was obtained using an amperometric gas difffusion electrode for oxygen and modifyin g its response by means of physically immobilized catalase enzyme, whi ch was superimposed on the teflon cap of the indicating electrode. Thr ee different methods were used to immobilize the enzyme. The results o btained in a series of experimental tests, carried out in water satura ted chloroform, using three different immobilization methods were comp ared and discussed on the basis of the response time, lifetime, sensit ivity, useful range and main analytical data.