LARGE-SCALE PREPARATION OF THE DELTA-10 FORM OF STAPHYLOKINASE BY IN-VITRO PROCESSING OF RECOMBINANT STAPHYLOKINASE WITH PURIFIED HUMAN PLASMINOGEN

The authors have developed a rapid and convenient method for purificat ion of a low molecular weight form (Delta 10) of the bacterial plasmin ogen activator, staphylokinase. Recombinant staphylokinase is expresse d in Escherichia coli, with an amino terminal extension that facilitat ed purification by immobilized metal-affinity chromatography. Purified staphylokinase is treated with human plasminogen, and the resulting t runcated form is purified using a combination of immobilized metal aff inity chromatography and hydrophobic interaction chromatography. Purif ied protein is characterized by amino terminal sequencing and in vitro plasminogen activation assay.