IMMOBILIZATION OF GLUCOSE-ISOMERASE AND ITS APPLICATION IN CONTINUOUSPRODUCTION OF HIGH-FRUCTOSE SYRUP

Bilayer glucose isomerase was immobilized in porous p-trimethylamine-p olystyrene (TMPS) beads, through a molecular deposition technique. Som e of the factors that influence the activity of immobilized glucose is omerase were optimized, with the enzyme concentration of 308 IU/mL, en zyme:matrix ratio of 924 IU/g wet carrier, and hexamethylene bis(trime thylammonium iodine) concentration of 15 mg/mL, giving the maximum cat alytic activity (2238 IU/g dry gel) of the immobilized bilayer glucose isomerase, retaining 68.5% of the initially added activity. The half- life of the immobilized bilayer glucose isomerase was approx 45 d at p H 8.5, 60 degrees C, with 50% (w/v) glucose as substrate. The specific productivity of the immobilized bilayer glucose isomerase was 223 g d ry D-glucose/g dry immobilized enzyme per day.