COUPLING TERMINATION OF TRANSCRIPTION TO MESSENGER-RNA MATURATION IN YEAST

The direct association between messenger RNA (mRNA) 3'-end processing and the termination of transcription was established for the CYC1 gene of Saccharomyces cerevisiae. The mutation of factors involved in the initial cleavage of the primary transcript at the poly(A) site (RNA 14 , RNA 15, and PCF11) disrupted transcription termination at the 3' end of the CYC1 gene. In contrast, the mutation of factors involved in th e subsequent polyadenylation step (PAP1, FIP1, and YTH1) had little ef fect. Thus, cleavage factors link transcription termination of RNA pol ymerase II with pre-mRNA 3'-end processing.