P. Douillet, DISINFECTION OF ROTIFER CYSTS LEADING TO BACTERIA-FREE POPULATIONS, Journal of experimental marine biology and ecology, 224(2), 1998, pp. 183-192
A reliable method was developed to disinfect cysts of the salt water r
otifers Brachionus plicatilis Muller and B. rotundiformis Tschugunoff.
The method leads to the production of axenic populations for both spe
cies of rotifers. The technique takes only a few minutes and requires
very few manipulations, so the possibility of bacterial contamination
is reduced. The microbiota that coats rotifer cysts was found to produ
ce no adverse effects on hatching efficiency. (C) 1998 Elsevier Scienc
e B.V.