T. Inoue et al., LOSS OF HETEROZYGOSITY ON CHROMOSOME 18Q IN COHESIVE-TYPE GASTRIC-CANCER IS ASSOCIATED WITH TUMOR PROGRESSION AND POOR-PROGNOSIS, Clinical cancer research, 4(4), 1998, pp. 973-977
Although loss of heterozygosity (LOH) on chromosome 18q is frequently
found in gastric cancer, the clinical significance of this abnormality
has not been well documented. We examined LOH on chromosome 18q22-23
in DNA extracted from the tissues of gastric cancer patients using the
PCR-based dinucleotide repeat assay with two microsatellite markers,
D18S61 and D18S58. We investigated LOH in 100 samples of DNA extracted
from formalin-fixed, paraffin-embedded tissues of cohesive-type gastr
ic cancer patients operated on between 1984 and 1993. Thirty-two of 83
informative cases (39%) showed LOH on chromosome 18q22-23 at one or t
wo loci, The LOH correlated significantly with serosal invasion of the
tumor (P = 0.004) and hematogenous recurrence (P = 0.035), In 60 case
s who were cured, the 5-year survival rate in patients with LOH (54%)
was lower than that in patients without LOH (81%; P = 0.019), These re
sults suggest that 18q22-23 LOH in cohesive gastric cancer is associat
ed with tumor progression and a patient's poor prognosis.