Lh. Wang et al., SENSITIVITY OF ANTICANCER DRUGS IN SAOS-2 CELLS TRANSFECTED WITH MUTANT P53 VARIED WITH MUTATION POINT, Anticancer research, 18(1A), 1998, pp. 321-325
A human osteosarcoma cell line, Saos-2, which is devoid of endogenous
p53 gene, and clones of Saos-2 cells, which were transfected with wild
type p53 or mutant p53 genes (123A, 143A, 175H and 273H), were observ
ed for their surviving fraction after treatment with the commonly used
anticancer drugs, cisplatin (CDDP), nimustine (ACNU), adriamicin (ADR
) and bleomicin (BLM). The transfectants of the mutant 143A, 175H and
273H were significantly more resistant to CDDP than the transfectant o
f pOPI3 (expression plasmid only). The transfectants of the wild type
p53 and mutant 123A were significantly more sensitive to ACNU than the
transfectant of pOPI3. The transfectant of mutant 123A was more sensi
tive to ADR than the transfectant of pOPI3. There was no significant d
ifference in sensitivity to BLM between Saos-2 and all kinds of transf
ectants. Thus the sensitivity of the cells to anticancer drugs varied
with the mutation point of the p53 gene.