EFFECT OF GLUCOSAMINYL MURAMYL DIPEPTIDE ON CELL-MEDIATED IMMUNE-RESPONSE AGAINST FOWL-POX

Role of glucosaminyl muramyl dipeptide (GMDP) on cell-mediated immune response (CMIR) in chicken vaccinated with fowl-pox vaccine was studie d using leucocyte migration inhibition test (LMIT) and skin reactive f actor (SRF). Mean per cent migration inhibition (% MI) was highest on 21 day post-vaccination with mean values of 65.00 in fowlpox vaccinate d group and 70.25 in GMDP and fowl-pox vaccinated group. Higher value of MI in GMDP and fowl-pox vaccinated group indicates immunostimulator y effect of GMDP on CMIR. An intense SRF was detected at 24 hr postino culation of lymphokines indicating delayed type hypersensitivity react ion. Highest SRF values of 7.2 mm was found on 21 st day post-vaccinat ion with GMDP and fowl-pox in comparison to the value of 5.2 mm in fow l-pox group giving an evidence of immunostimulatory effect of GMDP on cell-mediated immune response. Histopathological examinations of guine apig skin sections revealed maximum cellular reactions at 24 hr post-i noculation of lymphokines. Oedema with few polymorphonuclear cells and predominant infiltration of mononuclear cells gives evidence of delay ed type of hypersensitivity reactions indicating involvement of cell-m ediated immune response.