HPLC METHOD FOR THE DETERMINATION OF ORNITHINE CARBAMYL TRANSFERASE-ACTIVITY IN HUMAN SERUM

A rapid and sensitive method has been developed for the determination of ornithyne carbamyl transferase (OCT) based on the separation and UV detection by ion-pair reversed phase high performance liquid chromato graphy following on an reaction mixture. The method is based on the me asurement of L-citrulline formed from L-ornithine and carbamyl phospha te. The reaction conditions for the assay of OCT in serum samples were examined. The absence of assay additional reactions or sample pretrea tment, makes this method more advanced compared with the other ones an d more suitable for the routine determination of OCT in human serum sa mple.