THE LIST OF CYTOPLASMIC RIBOSOMAL-PROTEINS OF SACCHAROMYCES-CEREVISIAE

Screening of the complete genome sequence from the yeast Saccharomyces cerevisiae has enabled us to compile a complete list of the genes enc oding cytoplasmic ribosomal proteins in this organism. Putative riboso mal protein genes were selected primarily on the basis of the sequence similarity of their products with ribosomal proteins from other eukar yotic organisms, in particular the rat. These genes were subsequently screened for typical yeast rp-gene characteristics, viz. (1) a high co don adaptation index; (2) their promoter structure and (3) their respo nses to changes in growth conditions. The yeast genome appears to carr y 78 different genes, of which 59 are duplicated, encoding 32 differen t small-subunit and 46 large-subunit proteins. A new nomenclature for these ribosomal proteins is proposed. (C) 1998 John Wiley & Sons, Ltd.