A SATELLITE DNA ELEMENT-SPECIFIC FOR ROE DEER (CAPREOLUS-CAPREOLUS)

An abundant tandem repetitive DNA segment (CCsatIII) with a repeat uni t of 2.2 kb has been found in the genome of roe deer (Capreolus capreo lus). It accounts for approximately 5%-10% of the genome and is only p resent in the two species of the genus Capreolus. The sequence has no similarity or common motifes with other deer satellite DNAs and there is no internal repeat structure, A 93 bp fragment is homologous to a b ovine repeat. Fluorescent in situ hybridisation showed a predominant c entromeric staining of most chromosomes accompanied by a weak intersti tial staining of the same chromosomes. On Southern blots, CCsatIII pro bes do not discriminate between the closely related Capreolus species.