COMPARISON OF 2 TYPES OF CARRIERS FOR THE PRODUCTION OF LIGNINOLYTIC ENZYMES BY PHANEROCHAETE-CHRYSOSPORIUM DURING SOLID-STATE CULTURE CONDITIONS

An inert carrier (polyurethane foam) and a non inert carrier (corn cob ) were used to study ligninolytic enzymes produced by Phanerochaete ch rysosporium BKM-F-1767 (ATCC 24725) during solid state culture conditi ons. The polyurethane foam is used by the fungus only as an anchorage system. However the corn cob is used as a substrate (carbon source), t oo. The optimal conditions (culture medium, environmental conditions) and the characteristics of the support (diametre, porosity and free li quid) were previously determined by using polyurethane foam as a carri er. The results obtained were applied to the corn cob, determining the optimal conditions. When polyurethane foam was used as a carrier MnP activities higher than 200 U/L were achieved whereas employing corn co b as a substrate MnP activities don't surpass of 96 U/L.