HEME-CONTAINING HYDROPEROXIDE TEST SYSTEMS FOR INHIBITORS OF FREE-RADICAL REACTIONS

Authors
METELITSA DI RUS OB PUCHKAEV AV
Citation
Di. Metelitsa et al., HEME-CONTAINING HYDROPEROXIDE TEST SYSTEMS FOR INHIBITORS OF FREE-RADICAL REACTIONS, Russian journal of applied chemistry, 70(10), 1997, pp. 1629-1636
Citations number
28
Journal title
Russian journal of applied chemistry → ACNP
ISSN journal
10704272
Volume
70
Issue
10
Year of publication
1997
Pages
1629 - 1636
Database
ISI
SICI code
1070-4272(1997)70:10<1629:HHTSFI>2.0.ZU;2-Y
Abstract
Rates of initiation of active oxygen-containing radicals in test syste ms consisting of hemin (methemalbumin) and tert-butyl hydroperoxide or H2O2 are estimated using o-phenylenediamine and 3,3',5,5'-tetramethyl benzidine as accepters of free radicals. The criterion of inhibitor ef ficiency is the ratio k(cat)/K-m (1 mol(-1) s(-1)), determined from th e Michaelis-Menten dependences for growing concentrations of inhibitor s being oxidized.