Ja. Bibbs et al., THE CONTRIBUTIONS OF SECONDARY PROTON BRIDGES TO THE CATALYTIC POWER OF THE SERINE PROTEASES, Berichte der Bunsengesellschaft fur Physikalische Chemie, 102(3), 1998, pp. 573-579
Citations number
37
Categorie Soggetti
Chemistry Physical
Journal title
Berichte der Bunsengesellschaft fur Physikalische Chemie
The curvature of proton-inventory functions (rate constants in mixture
s of protium and deuterium oxides) for hydrolysis of substrates of var
ying structure by trypsins from four different sources has been used a
s a signal of transition-state stabilization by a short, strong hydrog
en bonds (''low-barrier hydrogen bonds'') at the histidine-aspartate s
ite of the catalytic triad. Previous estimates suggested contributions
as large as 85 kJ mol(-1) but the current findings are consistent wit
h contributions of no more than a few kJ mol(-1).