CHROMOSOMAL LOCATION OF PCR FRAGMENTS AS A SOURCE OF DNA MARKERS LINKED TO ALUMINUM TOLERANCE GENES IN RYE

To identify and locate rye DNA sequences homologous to three wheat c-D NAs (wali1, wali2 and wali5) whose expression is induced by aluminium (Al) stress, we designed three pairs of specific primers, They were us ed in the amplification of genomic DNA from wheat-rye disomic addition lines. The wali2 pair of primers amplified a 878-bp rye DNA fragment (rali2) located on chromosomes 4R and 7R that showed 79.37% homology w ith the corresponding wheat c-DNA. RAPD fragments were also used as ge netic markers. We located 22 different RAPDs distributed on II differe nt rye chromosome arms using wheat-rye disomic and ditelocentric addit ion lines. Thirteen of these markers were located on the chromosomes 3 R, 4R and 6R, which also carry aluminium-tolerance genes. The OPA08(41 5) and OPR01(600) RAPD markers, located on the 6RL and 6RS chromosome arms, respectively. were converted to SCAR markers (SCA08(415) and SCR 01(600)) and linked to Alt1 gene (SCR01(600)-2.1 cM-Alt1-33.5 cM-SCA08 (415)). We propose that the chromosomal location of RAPDs and SCARs us ing wheat-rye addition lines is a source of DNA markers linked to alum inium-tolerance loci and offers a valuable strategy in marker-assisted selection for the introgression of tolerance genes in wheat.